Diagnostics using isothermal DNA amplification (LAMP, RT-LAMP)

Protean Ltd. has developed technology for a simple detection of various pathogens and specific DNA and RNA sequences based on isothermal amplification. The technology profits from in-house production of amplification enzymes BstI DNA polymerase and ProRT Reverse Transcriptase and fully complies with CE IVD certification standards. A patent application was filed for the technological solution of this diagnostics.

Major advantages:

Easy on laboratory equipment and staff - it can be done in any laboratory, no PCR cycler required.

Results are available within one hour - with the ability to read results with the naked eye or mobile (also spectrophotometrically).

Huge test throughput - hundreds of samples can be analyzed at once.

Significant cost reduction over traditional PCR assays while maintaining the same sensitivity and specificity.

Commercial application - RT-LAMP Assay SARS-CoV2

The AUMED test kit RT-LAMP Assay SARS-CoV2 is intended for diagnostics of SARS-CoV-2 virus in isolated viral RNA samples - for 45 samples. However, this test can also detect primary samples without RNA isolation.

Test principle

RT-LAMP (reverse transcription and loop-mediated isothermal amplification) allows amplification of nucleic acids with a high specificity, efficiency and speed under isothermal conditions - ie. at constant temperature, achievable in incubators, hybridization ovens or dry blocks. The results are reliably read based on the color change by naked eye. The test includes detection of SARS-CoV2 virus and detection of internal control (IC) for correct sampling and processing.

Kit contents

Necessary material not supplied with the kit

Test sensitivity