|Purity||>95% by SDS PAGE|
Substrate protein for quantitative determination and monitoring of Caspase-3 specific activity based on fluorescent energy transfer (FRET). Suitable for high-throughput applications.
The 60 kDa FRET substrate protein is composed by two fluorescent proteins linked with Caspase-3 recognition sequence DEVD. The substrate is specifically cleaved to fluorescent monomers, which results in quantitative decrease of fluorescent intensity at 580-650 nm (emission). The excitation range of the substrate is 490-515 nm.
Protease activity control and monitoring. High-throughput screening of Caspase-3 protease variants. This substrate is manufactured in certified laboratory environment and could be used in GMP certified downstream processes.
Affinity chromatography, size exclusion chromatography, desalting.
0.5 mg/ml, 10mM Tris pH7,5, 50mM KCl
Caspase-3 protease recognition sequence.
-80°C, aliquot to avoid repeated freezing and thawing.
Analyte specific reagent (ASR) manufactured under ISO 13485