Recombinant proteins Protease substrates FRET HRV-3C substrate
Print page

FRET HRV-3C substrate (Prescission fluorescent substrate)

Catalog # 2614
Type Recombinant
Source E. coli
Species Human
Tag His6
Form Liquid
Purity >95% by SDS PAGE
Shipping Ice pack
See full characteristics ↓

In stock, ready for shipping in 24 hours
Need help or bulk amount? Feel free to contact us! More contacts

FRET HRV-3C substrate


Substrate protein for quantitative determination and monitoring of human rhinovirus 3C (HRV-3C, Prescission) protease specific activity based on fluorescent energy transfer (FRET). Suitable for high-throughput applications.


The 60 kDa FRET substrate protein is composed by two fluorescent proteins linked with Caspase-3 recognition sequence LEVLFQ|GP. The substrate is specifically cleaved to fluorescent monomers, which results in quantitative decrease of fluorescent intensity at 580-650 nm (emission). The excitation range of the substrate is 490-515 nm.


Protease activity control and monitoring. High-throughput screening of Prescission (HRV-3C) protease variants. This substrate is manufactured in certified laboratory environment and could be used in GMP certified downstream processes.

Purification method

Affinity chromatography, size exclusion chromatography, desalting.


0.5 mg/ml, 10mM Tris pH7,5, 50mM KCl


HRV-3C protease recognition sequence.


-80°C, aliquot to avoid repeated freezing and thawing.

Analyte specific reagent (ASR) manufactured under ISO 13485

Country of origin: Czech Republic

Do you need a specific product? Try custom protein production