Introduction
Endolysins are hydrolytic enzymes produced by bacteriophages in order to cleave the host's cell wall during the final stage of the lytic cycle. They are highly evolved enzymes able to cleave peptidoglycan (murein), the main component of bacterial cell wall, which allows the release of progeny virions from the lysed cell. They are highly species specific, leaving non-target bacteria intact.
Description
Recombinant CHAP fragment of phage Φ812 catalytic domain
Application
Anti-Staphylococcus aureus activities, Turbidity-reduction assay, plaque assay, various anti-pathogen studies
Purification method
Affinity chromatography
Formulation
50mM Tris pH 7,5; 150 mM NaCl, 1 mM EDTA; 5 mM DTT, 50% glycerol, may contain traces of imidazol
Specificity
Specifically cleave peptidoglycan in cell wall leading to cell lysis.
Storage
-80°C, aliquot to avoid repeated freezing and thawing.
