Introduction
Our Taq DNA Polymerase is a genetically modified version of wt Taq DNA polymerase optimized for all standard PCR applications. It provides higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerases. Taq DNA Polymerase could be used with the same cycling conditions as conventional Taq DNA polymerase with minimum optimization. It is supplied with optimized Taq 10x buffer, which includes 20 mM MgCl2.
Description
Taq DNA polymerase is a highly pure recombinant enzyme with a high processivity. It contains His6 tag and could be efficiently removed for downstream applications. It is supplied with proprietary Taq 10x buffer, which includes KCl, (NH4)2SO4, 2mg/ml BSA and 20 mM MgCl2. The concentration is 5 U/ul.
Typical reaction setup (25-45 cycles):
Initial denaturation: 95°C 1-3 min,
Denaturation: 95°C 30 s,
Annealing: Tm-5°C 30 s,
Extension*: 72°C 1 min,
Final Extension: 72°C 5-15 min.
*The recommended extension time is 1 min for PCR products up to 2 kb. For longer products, the extension time should be prolonged by 1 min/kb.
Application
PCR reactions, PCR labeling - effectively incorporates modified dUTP (Biotin, Digoxigenin, Cy5, Cy3), PCR Mutagenesis, Fill-in reactions, Primer extension, T/A cloning
Purification method
Affinity chromatography
Formulation
Storage buffer: 50mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 0.1mM EDTA, 0.5% (v/v) Tween 20, 50% (v/v) glycerol
Specificity
Product overhang: 3'-A
Storage
Store at -20°C
