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Tick-Taq DNA polymerase (Taq polymerase)

Catalog # 1609
Type Recombinant
Source E. coli
Species Thermus aquaticus
Tag His6
Form 50% Glycerol soluttion + Buffer 10x
 
Purity >95% by SDS PAGE
Shipping Ice pack
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Introduction

Tick-Taq DNA Polymerase is a genetically modified version of Taq DNA polymerase optimized for all standard PCR applications. It provides higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerases. Tick-Taq DNA Polymerase could be used with the same cycling conditions as conventional Taq DNA polymerase with minimum optimization. It is supplied with optimized Tick-Taq 10x buffer, which includes 20 mM MgCl2.

Description

Tick-Taq DNA polymerase is a highly pure recombinant enzyme with a high processivity. It contains His6 tag and could be efficiently removed for downstream applications. It is supplied with proprietary Tick-Taq 10x buffer, which includes KCl, (NH4)2SO4 and 20 mM MgCl2. The concentration is 5 U/ul. Typical reaction setup (25-45 cycles): Initial denaturation: 95°C 1-3 min, Denaturation: 95°C 30 s, Annealing: Tm-5°C 30 s, Extension*: 72°C 1 min, Final Extension: 72°C 5-15 min. *The recommended extension time is 1 min for PCR products up to 2 kb. For longer products, the extension time should be prolonged by 1 min/kb.

Application

PCR reactions, PCR labeling - effectively incorporates modified dUTP (Biotin, Digoxigenin, Cy5, Cy3), PCR Mutagenesis, Fill-in reactions, Primer extension, T/A cloning

Purification method

Affinity chromatography

Formulation

Storage buffer: 50mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT, 0.1mM EDTA, 0.5% (v/v) Tween 20, 50% (v/v) glycerol

Specificity

Product overhang: 3'-A

Storage

Store at -20°C


For research use only. Not for use in diagnostic procedures.


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