IRF-3 (Interferon regulatory factor 3)

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Introduction

IRF-3 is a member of the interferon regulatory transcription factor (IRF) family.

Description

IRF-3 was originally discovered as a homolog of IRF-1 and IRF-2. It has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF-3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CBP known also as CREB binding protein. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 plays an important role in the innate immune system's response to viral infection.

Application

Western blot, ELISA, Gel-shift assay, Pull-down assay (upon removal of GSH), protein arrays

Purification method

Affinity chromatography.

Formulation

Buffered solution containing 10mM glutathion. 0.25mg/ml, degradation products may appear upon thawing.

Specificity

Binds specific DNA elements.

Storage

-80°C