Introduction
IRF-3 is a member of the interferon regulatory transcription factor (IRF) family.
Description
IRF-3 was originally discovered as a homolog of IRF-1 and IRF-2. It has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. IRF-3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CBP known also as CREB binding protein. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 plays an important role in the innate immune system's response to viral infection.
Application
Western blot, ELISA, Gel-shift assay, Pull-down assay (upon removal of GSH), protein arrays
Purification method
Affinity chromatography.
Formulation
Buffered solution containing 10mM glutathion. 0.25mg/ml, degradation products may appear upon thawing.
Specificity
Binds specific DNA elements.
Storage
-80°C
