ProRT Reverse Transcriptase (MMLV-RT, Thermostable RT H-)

Introduction

Moloney Murine Leukemia Virus encoded sequence of reverse transcriptase was in silico optimized and produced in E.coli strain.

Description

Higly active recombinant enzyme with an increase thermal stability. RNAse H-minus fragment of reverse transcriptase suitable for all molecular techniques requiring reliable reverse transcriptase activity, such as RT-PCR and RT-LAMP assays. An increased thermal stability of this particular enzyme allow coupled reverse transcription at elevated temperatures up to 60°C, which results in an increased sensitivity and specificity of various detection techniques. The concentration is 200 U/ul.

Application

RT-LAMP, RT-PCR, RT-qPCR

Purification method

Affinity chromatography. The enzymes supplied by Protean Ltd. are manufactured in certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes.

Formulation

Storage buffer: 10mM Tris (pH 7,5), 50mM KCl, 1mM DTT, 0,1mM EDTA, 0,1% Triton, 50% glycerol. 10x Reaction Buffer: Proprietary optimized buffer.

Specificity

RNA

Storage

Store at -20°C