Pro3C HRV-3C Protease (Prescission, HRV-3C)

Introduction

Pro3C (HRV-3C, Prescission) protease is a genetically modified sequence-specific cysteine protease from Human Rhinovirus 3C (HRV3C). Due to its high sequence specificity it is frequently used for the cleavage of fusion proteins and removal of tags from recombinant proteins in vitro and in vivo.

Description

This recombinant protease is genetically improved version of the human rhinovirus 3C. It contains His6 tag located at the N-terminus of the protein, which allows it to be immobilized on Ni-based affinity resins and removed from the cleavage reaction. It is active over a wide range of temperatures (4-34°C). The optimum is 30°C, however, it is active at 4°C as well for temperature sensitive targets. The preferred recognition sequence is Leu-Glu-Val-Leu-Phe-Gln|Gly-Pro (LEVLFQ|GP). Please use our 3C substrate #1409 or FRET substrate as a positive control.

Application

Cleavage of affinity tags from fusion proteins after protein purification. Cleaves fusion proteins directly in solution or immobilized on affinity resins. The enzymes supplied by Protean Ltd. are manufactured in certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes.

Purification method

Affinity chromatography

Formulation

50 mM Tris pH 7.0, 150 mM NaCl, 1 mM EDTA, 1mM DTT, 50% glycerol

Specificity

Highly specific and active for its eight-amino acid sequence with minimal off-target effects. The activity depends on the type of target protein. The optimal amount of enzyme should be tested for each target protein.

Storage

-20°C, do not store at -80°C