|Purity||>96% by SDS-PAGE|
Endonuclease (Benzonase or Supernuclease) is nonspecific endonuclease isolated from Serratia marcescens. It is higly processive enzyme, which is active at various conditions and temperatures. It has no proteolytic activity and is therefore ideal tool for removing nucleic acids from protein samples. It is an cost-effective alternative to the nuclease sold under trade mark Benzonase®.
Nuclease digests both single and double stranded DNA and RNA. It has no sequence specificity and cleavage leads to oligos 2 to 5 bases short. This nuclease is an ideal tool for removal of nucleic acid contamination from purified proteins, commonly from recombinant DNA products, and reduction of viscosity of bacterial lysates for downstream steps enhancement.
Degradation of DNA upon cell lysis, viscosity reduction.
Supplied as liquid in 50% glycerol, 20mM Tris pH 8, 2mM MgCl2, 100mM NaCl, 15mM NaH2PO4, 80mM imidazole, 500 U/ul. One unit of the enzyme cleaves the amount of DNA causing an A260 change of 1.0 in 30 minutes at 37°C under standard assay conditions of 50 mM Tris-HCl (pH 8.0), 1 mM MgCl2, 100 ug/ml BSA, and 1 mg/ml sonicated salmon sperm DNA. The absorbance at 260 nm is determined following perchloric acid precipitation.
Nonspecifically cleaves both single and double strand DNA and RNA of any origin. Highly processive. Inhibited by >250 mM NaCl.
For research use only. Not for use in diagnostic procedures.