|Purity||>95% by SDS PAGE|
Moloney Murine Leukemia Virus encoded sequence of reverse transcriptase was in silico optimized and produced in E.coli strain.
Higly active recombinant enzyme with an increase thermal stability. RNAse H-minus fragment of reverse transcriptase suitable for all molecular techniques requiring reliable reverse transcriptase activity, such as RT-PCR and RT-LAMP assays. An increased thermal stability of this particular enzyme allow coupled reverse transcription at elevated temperatures up to 60°C, which results in an increased sensitivity and specificity of various detection techniques. The concentration is 200 U/ul.
RT-LAMP, RT-PCR, RT-qPCR
Affinity chromatography. The enzymes supplied by Protean Ltd. are manufactured in certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes.
Storage buffer: 10mM Tris (pH 7,5), 50mM KCl, 1mM DTT, 0,1mM EDTA, 0,1% Triton, 50% glycerol. 10x Reaction Buffer: Proprietary optimized buffer.
Store at -20°C
For research use only. Not for use in diagnostic procedures.