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ProRT Reverse Transcriptase (MMLV-RT, Thermostable RT H-)

Catalog # 1972
Type Recombinant
Source E. coli
Species MMLV
Tag His6
Form Liquid
 
Purity >95% by SDS PAGE
Shipping Ice pack
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ProRT Reverse Transcriptase

Introduction

Moloney Murine Leukemia Virus encoded sequence of reverse transcriptase was in silico optimized and produced in E.coli strain.

Description

Higly active recombinant enzyme with an increase thermal stability. RNAse H-minus fragment of reverse transcriptase suitable for all molecular techniques requiring reliable reverse transcriptase activity, such as RT-PCR and RT-LAMP assays. An increased thermal stability of this particular enzyme allow coupled reverse transcription at elevated temperatures up to 60°C, which results in an increased sensitivity and specificity of various detection techniques. The concentration is 200 U/ul.

Application

RT-LAMP, RT-PCR, RT-qPCR

Purification method

Affinity chromatography. The enzymes supplied by Protean Ltd. are manufactured in certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes.

Formulation

Storage buffer: 10mM Tris (pH 7,5), 50mM KCl, 1mM DTT, 0,1mM EDTA, 0,1% Triton, 50% glycerol. 10x Reaction Buffer: Proprietary optimized buffer.

Specificity

RNA

Storage

Store at -20°C


For research use only. Not for use in diagnostic procedures.


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