Recombinant proteins Enzymes Proteases Arctic-TEV Protease His6
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Arctic-TEV Protease His6 (Endopeptidase, ProTEV)

Catalog # 2279
Type Recombinant
Source E. coli
Species Tobacco etch virus
Tag His6
Form Liquid
Purity >95% by SDS PAGE
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Arctic-TEV Protease His6


TEV protease is genetically modified sequence-specific cysteine protease from Tobacco Etch Virus (TEV). It is a member of chymotrypsin-like proteases. Due to its high sequence specificity, it is frequently used for the cleavage of fusion proteins and removal of tags from recombinant proteins in vitro and in vivo.


Genetically modified version of the TEV protease with increased activity at 6°C. The activity of this enzyme at 6°C is comparable with the activities of competitor's TEV proteases at 30°C. It contains His6 tag located at the N-terminus of the protein, which allows it to be immobilized on Ni-based affinity resins and removed from the cleavage reaction. The preferred recognition sequence is the same ENLYFQ|S(G,A), but it cleaves also motives EXLYFQ|S(G,A), where X could be any amino acid residue (in parenthesis are alternative residues). Please use our TEV substrate #1409 as a positive control.


Cleavage of affinity tags from fusion proteins after protein purification. Cleaves fusion proteins directly in solution or immobilized on affinity resins. The enzymes supplied by Protean Ltd. are manufactured locally in a certified environment under ISO 9001 and ISO 13485 international standards, which fully qualifies them for a use in downstream GMP certified processes. More details here.

Purification method

Affinity chromatography


50mM Tris pH 7,5, 1 mM EDTA, 5 mM DTT, 40% glycerol


Highly specific and active for its seven-amino acid sequence with minimal off-target effects. Activity more than 20KU per mg of protein. The activity depends on the type of target protein. The optimal amount of enzyme should be tested for each target protein.


-20°C, do not store at -80°C

Analyte specific reagent (ASR) manufactured under ISO 13485

Country of origin: Czech Republic

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